Pistil Mother appears in the first World of Magatsu Mandala within the Midnight Channel. It will try to Silence the party along with using Ice and Wind spells. If left injured, it will heal itself or other Shadows. After being defeated, it may drop a Queen's Nail. Selling 4 Queen's Nails will unlock the Needle Spike weapon for Teddie in the shop.
In Golden, Pistil Mother appears in the 1st and 2nd floor of Magatsu Inaba, and Worlds 1 to 3 of Magatsu Mandala. It will try to Silence the party. It also has access to heavy single- and multi-target ice and wind spells. It may drop Angelite for trade at Shiroku Pub.
During a special screening in Hikari labyrinth, the group are trying to find something that Hikari had lost, and are eventually lead by the talking flowers to a house in a dead end, within which are two Reckless Okina and a Pistil Mother. After killing the shadows, Hikari finds what she was looking for, her most pride possession, a toy clapboard, which she then gives to group, as she wanted to thank them for giving her so many happy memories and giving her the courage to move forward in life.