Pistil Mother appears in the Harabah Block of Tartarus, from floors 191 to 200. They will attempt to heal other shadows or themselves when injured.
Pistil Mother appears in the first World of Magatsu Mandala within the Midnight Channel. It will try to Silence the party along with using Ice and Wind spells. If left injured, it will heal itself or other Shadows. After being defeated, it may drop a Queen's Nail. Selling 4 Queen's Nails will unlock the Needle Spike weapon for Teddie in the shop.
In Golden, Pistil Mother appears in the 1st and 2nd floor of Magatsu Inaba, and Worlds 1 to 3 of Magatsu Mandala. It will try to Silence the party. It also has access to heavy single- and multi-target ice and wind spells. It may drop Angelite for trade at Shiroku Pub.